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1.
Plant Phenomics ; 6: 0163, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38586218

RESUMO

Asian soybean rust (ASR) is one of the major diseases that causes serious yield loss worldwide, even up to 80%. Early and accurate detection of ASR is critical to reduce economic losses. Hyperspectral imaging, combined with deep learning, has already been proved as a powerful tool to detect crop diseases. However, current deep learning models are limited to extract both spatial and spectral features in hyperspectral images due to the use of fixed geometric structure of the convolutional kernels, leading to the fact that the detection accuracy of current models remains further improvement. In this study, we proposed a deformable convolution and dilated convolution neural network (DC2Net) for the ASR detection. The deformable convolution module was used to extract the spatial features, while the dilated convolution module was applied to extract features from the spectral dimension. We also adopted the Shapley value and the channel attention methods to evaluate the importance of each wavelength during decision-making, thereby identifying the most contributing ones. The proposed DC2Net can realize early asymptomatic detection of ASR even when visual symptoms have not appeared. The results of the experiment showed that the detection performance of DC2Net dominated state-of-the-art methods, reaching an overall accuracy at 96.73%. Meanwhile, the experimental result suggested that the Shapley Additive exPlanations method was able to extract feature wavelengths correctly, thereby helping DC2Net achieve reasonable performance with less input data. The research result of this study could provide early warning of ASR outbreak in advance, even at the asymptomatic period.

2.
Spectrochim Acta A Mol Biomol Spectrosc ; 310: 123895, 2024 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-38262294

RESUMO

Using optical density at 600 nm (OD600) to measure the microbial concentration is a popular approach due to its advantages like quick response and non-destructive. However, the OD600 measurement might be affected by the metabolic pigment, and it would become invalid when the solution dilution is insufficient. To overcome these issues, we proposed to adopt a more robust wavelength at 890 nm to quantify the attenuation of transmission light. After selecting this light source, we designed the light path and the circuit of the online monitoring device. Meanwhile, the random forest algorithm was introduced for temperature compensation and improving the stability of the device. This device was verified by monitoring the microbial concentration of four strains (Yeast, Bacillus, Arthrobacter, and Escherichia coli). The experimental result suggested that the mean absolute percentage error reached 4.11 %, 4.28 %, 4.49 %, and 4.53 % respectively, which is helpful to improve the accuracy of microbial concentration measurement.


Assuntos
Bacillus , Escherichia coli/metabolismo , Temperatura
3.
Cancers (Basel) ; 15(23)2023 Nov 21.
Artigo em Inglês | MEDLINE | ID: mdl-38067200

RESUMO

Pancreatic ductal adenocarcinoma (PDAC) presents a critical global health challenge, and early detection is crucial for improving the 5-year survival rate. Recent medical imaging and computational algorithm advances offer potential solutions for early diagnosis. Deep learning, particularly in the form of convolutional neural networks (CNNs), has demonstrated success in medical image analysis tasks, including classification and segmentation. However, the limited availability of clinical data for training purposes continues to represent a significant obstacle. Data augmentation, generative adversarial networks (GANs), and cross-validation are potential techniques to address this limitation and improve model performance, but effective solutions are still rare for 3D PDAC, where the contrast is especially poor, owing to the high heterogeneity in both tumor and background tissues. In this study, we developed a new GAN-based model, named 3DGAUnet, for generating realistic 3D CT images of PDAC tumors and pancreatic tissue, which can generate the inter-slice connection data that the existing 2D CT image synthesis models lack. The transition to 3D models allowed the preservation of contextual information from adjacent slices, improving efficiency and accuracy, especially for the poor-contrast challenging case of PDAC. PDAC's challenging characteristics, such as an iso-attenuating or hypodense appearance and lack of well-defined margins, make tumor shape and texture learning challenging. To overcome these challenges and improve the performance of 3D GAN models, our innovation was to develop a 3D U-Net architecture for the generator, to improve shape and texture learning for PDAC tumors and pancreatic tissue. Thorough examination and validation across many datasets were conducted on the developed 3D GAN model, to ascertain the efficacy and applicability of the model in clinical contexts. Our approach offers a promising path for tackling the urgent requirement for creative and synergistic methods to combat PDAC. The development of this GAN-based model has the potential to alleviate data scarcity issues, elevate the quality of synthesized data, and thereby facilitate the progression of deep learning models, to enhance the accuracy and early detection of PDAC tumors, which could profoundly impact patient outcomes. Furthermore, the model has the potential to be adapted to other types of solid tumors, hence making significant contributions to the field of medical imaging in terms of image processing models.

4.
Plant Methods ; 19(1): 116, 2023 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-37907992

RESUMO

BACKGROUND: Although previous studies on the droplet deposition behaviour of rice leaves have modelled the leaves as flat surface structures, their curved surface structures actually have a significant effect on droplet deposition. RESULTS: In this paper, the statistical distribution of the coordinate parameters of rice leaves at the elongation stage was determined, computational fluid dynamics (CFD) simulation models of droplet impact on rice leaves with different curvature radii were built, and the effect of leaf curvature radius on the deposition behaviour and spreading diameter of droplets on rice leaves was studied using validated simulation models. The results showed that the average relative errors of the CFD simulation models were in the range of 2.23-9.63%. When the droplets struck the rice leaves at a speed of 4 m/s, the 50 µm droplets did not bounce within the curvature radii of 25-120 cm, the maximum spreading diameters of 200 and 500 µm droplets that just adhered to the leaves were 287 and 772 µm, respectively. The maximum spreading diameters of 50, 200, and 500 µm droplets that just split were 168, 636, and 1411 µm, respectively. As the curvature radii of the leaves increased, the maximum spreading diameter of the droplets gradually decreased, and droplet bouncing was more likely to occur. However, a special case in which no significant change in the maximum spreading diameter arose when 50 µm droplets hit a leaf with a curvature radius exceeding 50 cm. CONCLUSION: Splitting generally occurred for large droplets with a small curvature radius and small tilt angle; bouncing generally occurred for large droplets with a large curvature radius and large tilt angle. When the droplet was small, the deposition behaviour was mostly adhesion. The change in spreading diameter after stabilisation was similar to the change in maximum spreading diameter, where the spreading diameter after stabilisation greatly increased after droplet splitting. This paper serves as a reference for the study of pesticide droplet deposition and its application in rice-plant protection.

5.
medRxiv ; 2023 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-37961101

RESUMO

Addressing the significant level of variability exhibited by pancreatic cancer necessitates the adoption of a systems biology approach that integrates molecular data, biological properties of the tumors, and clinical features of the patients. In this study, a comprehensive multi-omics methodology was employed to examine a distinctive collection patient dataset containing rapid autopsy tumor and normal tissue samples as well as longitudinal imaging with a focus on pancreatic cancer. By performing a whole exome sequencing analysis on tumor and normal tissues to identify somatic gene variants and a radiomics feature analysis to tumor CT images, the genome-wide association approach established a connection between pancreatic cancer driver genes and relevant radiomics features, enabling a thorough and quantitative assessment of the heterogeneity of pancreatic tumors. The significant association between sets of genes and radiomics features revealed the involvement of genes in shaping tumor morphological heterogeneity. Some results of the association established a connection between the molecular level mechanism and their outcomes at the level of tumor structural heterogeneity. Because tumor structure and tumor structural heterogeneity are related to the patients' overall survival, patients who had pancreatic cancer driver gene mutations with an association to a certain radiomics feature have been observed to experience worse survival rates than cases without these somatic mutations. Furthermore, the outcome of the association analysis has revealed potential gene mutations and radiomics feature candidates that warrant further investigation in future research endeavors.

6.
Artigo em Inglês | MEDLINE | ID: mdl-37027272

RESUMO

Natural language moment localization aims to localize the target moment that matches a given natural language query in an untrimmed video. The key to this challenging task is to capture fine-grained video-language correlations to establish the alignment between the query and target moment. Most existing works establish a single-pass interaction schema to capture correlations between queries and moments. Considering the complex feature space of lengthy video and diverse information between frames, the weight distribution of information interaction flow is prone to dispersion or misalignment, which leads to redundant information flow affecting the final prediction. We address this issue by proposing a capsule-based approach to model the query-video interactions, termed the Multimodal, Multichannel, and Dual-step Capsule Network (M 2 DCapsN), which is derived from the intuition that "multiple people viewing multiple times is better than one person viewing one time." First, we introduce a multimodal capsule network, replacing the single-pass interaction schema of "one person viewing one time" with the iterative interaction schema of "one person viewing multiple times", which cyclically updates cross-modal interactions and modifies potential redundant interactions via its routing-by-agreement. Then, considering that the conventional routing mechanism only learns a single iterative interaction schema, we further propose a multichannel dynamic routing mechanism to learn multiple iterative interaction schemas, where each channel performs independent routing iteration to collectively capture cross-modal correlations from multiple subspaces, that is", multiple people viewing." Moreover, we design a dual-step capsule network structure based on the multimodal, multichannel capsule network, bringing together the query and query-guided key moments to jointly enhance the original video, so as to select the target moments according to the enhanced part. Experimental results on three public datasets demonstrate the superiority of our approach in comparison with state-of-the-art methods, and comprehensive ablation and visualization analysis validate the effectiveness of each component of the proposed model.

7.
Nat Commun ; 14(1): 1444, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36922495

RESUMO

With the advancement of global civilisation, monitoring and managing dumpsites have become essential parts of environmental governance in various countries. Dumpsite locations are difficult to obtain in a timely manner by local government agencies and environmental groups. The World Bank shows that governments need to spend massive labour and economic costs to collect illegal dumpsites to implement management. Here we show that applying novel deep convolutional networks to high-resolution satellite images can provide an effective, efficient, and low-cost method to detect dumpsites. In sampled areas of 28 cities around the world, our model detects nearly 1000 dumpsites that appeared around 2021. This approach reduces the investigation time by more than 96.8% compared with the manual method. With this novel and powerful methodology, it is now capable of analysing the relationship between dumpsites and various social attributes on a global scale, temporally and spatially.

8.
Sensors (Basel) ; 23(4)2023 Feb 08.
Artigo em Inglês | MEDLINE | ID: mdl-36850487

RESUMO

Leaf numbers are vital in estimating the yield of crops. Traditional manual leaf-counting is tedious, costly, and an enormous job. Recent convolutional neural network-based approaches achieve promising results for rosette plants. However, there is a lack of effective solutions to tackle leaf counting for monocot plants, such as sorghum and maize. The existing approaches often require substantial training datasets and annotations, thus incurring significant overheads for labeling. Moreover, these approaches can easily fail when leaf structures are occluded in images. To address these issues, we present a new deep neural network-based method that does not require any effort to label leaf structures explicitly and achieves superior performance even with severe leaf occlusions in images. Our method extracts leaf skeletons to gain more topological information and applies augmentation to enhance structural variety in the original images. Then, we feed the combination of original images, derived skeletons, and augmentations into a regression model, transferred from Inception-Resnet-V2, for leaf-counting. We find that leaf tips are important in our regression model through an input modification method and a Grad-CAM method. The superiority of the proposed method is validated via comparison with the existing approaches conducted on a similar dataset. The results show that our method does not only improve the accuracy of leaf-counting, with overlaps and occlusions, but also lower the training cost, with fewer annotations compared to the previous state-of-the-art approaches.The robustness of the proposed method against the noise effect is also verified by removing the environmental noises during the image preprocessing and reducing the effect of the noises introduced by skeletonization, with satisfactory outcomes.


Assuntos
Produtos Agrícolas , Grão Comestível , Redes Neurais de Computação , Folhas de Planta , Esqueleto
9.
Water Res ; 233: 119745, 2023 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-36812816

RESUMO

Groundwater is a crucial resource across agricultural, civil, and industrial sectors. The prediction of groundwater pollution due to various chemical components is vital for planning, policymaking, and management of groundwater resources. In the last two decades, the application of machine learning (ML) techniques for groundwater quality (GWQ) modeling has grown exponentially. This review assesses all supervised, semi-supervised, unsupervised, and ensemble ML models implemented to predict any groundwater quality parameter, making this the most extensive modern review on this topic. Neural networks are the most used ML model in GWQ modeling. Their usage has declined in recent years, giving rise to more accurate or advanced techniques such as deep learning or unsupervised algorithms. Iran and the United States lead the world in areas modeled, with a wealth of historical data available. Nitrate has been modeled most exhaustively, targeted by nearly half of all studies. Advancements in future work will be made with further implementation of deep learning and explainable artificial intelligence or other cutting-edge techniques, application of these techniques for sparsely studied variables, the modeling of new or unique study areas, and the implementation of ML techniques for groundwater quality management.


Assuntos
Monitoramento Ambiental , Água Subterrânea , Monitoramento Ambiental/métodos , Inteligência Artificial , Redes Neurais de Computação , Aprendizado de Máquina
10.
IEEE Trans Neural Netw Learn Syst ; 34(1): 228-242, 2023 01.
Artigo em Inglês | MEDLINE | ID: mdl-34255636

RESUMO

Sarcasm is a sophisticated construct to express contempt or ridicule. It is well-studied in multiple disciplines (e.g., neuroanatomy and neuropsychology) but is still in its infancy in computational science (e.g., Twitter sarcasm detection). In contrast to previous methods that are usually geared toward a single discipline, we focus on the multidisciplinary cross-innovation, i.e., improving embryonic sarcasm detection in computational science by leveraging the advanced knowledge of sarcasm cognition in neuroanatomy and neuropsychology. In this work, we are oriented toward sarcasm detection in social media and correspondingly propose a multimodal, multi-interactive, and multihierarchical neural network ( M3N2 ). We select Twitter, image, text in image, and image caption as the input of M3N2 since the brain's perception of sarcasm requires multiple modalities. To reasonably address the multimodalities, we introduce singlewise, pairwise, triplewise, and tetradwise modality interactions incorporating gate mechanism and guide attention (GA) to simulate the interactions and collaborations of involved regions in the brain while perceiving multiple modes. Specifically, we exploit a multihop process for each modality interaction to extract modal information multiple times using GA for obtaining multiperspective information. Also, we adopt a two-hierarchical structure leveraging self-attention accompanied by attention pooling to integrate multimodal semantic information from different levels mimicking the brain's first- and second-order comprehensions of sarcasm. Experimental results show that M3N2 achieves competitive performance in sarcasm detection and displays powerful generalization ability in multimodal sentiment analysis and emotion recognition.


Assuntos
Mídias Sociais , Humanos , Redes Neurais de Computação , Cognição , Encéfalo , Compreensão
11.
Pest Manag Sci ; 79(1): 402-414, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36175385

RESUMO

BACKGROUND: Currently, the variable-rate application (VA) of agrochemicals on fruit trees is based on canopy volume and biomass. The canopy volume has a significant relationship with disease resistance and degree of disease incidence. Therefore, this study proposes a VA method that uses deep convolutional neural networks for real-time recognition of disease spots on pear trees. Furthermore, it specifies the limitations and application scenarios of the disease spot recognition. Field performance tests were conducted to verify the performance of the proposed VA system. RESULTS: The results showed a mean average precision, precision, and recall of 87.42%, 83.76%, and 87.23%, respectively. The spot recognition rate was 81.3% when the canopy sampling distance, spot diameter, and canopy porosity were 1.2 m, 4-8 mm, and 55.76%, respectively. The results indicate that the proposed VA system saved 51.9% spray volume compared to conventional methods while ensuring quality. CONCLUSION: Compared to the traditional constant rate model, the proposed VA technology based on real-time disease spot identification can reduce spraying in nondiseased areas, thereby abandoning the previous saturation application practice and significantly reducing pesticide use. © 2022 Society of Chemical Industry.

12.
Plant Methods ; 18(1): 126, 2022 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-36443862

RESUMO

BACKGROUND: Our understanding of the physiological responses of rice inflorescence (panicle) to environmental stresses is limited by the challenge of accurately determining panicle photosynthetic parameters and their impact on grain yield. This is primarily due to the lack of a suitable gas exchange methodology for panicles and non-destructive methods to accurately determine panicle surface area. RESULTS: To address these challenges, we have developed a custom panicle gas exchange cylinder compatible with the LiCor 6800 Infra-red Gas Analyzer. Accurate surface area measurements were determined using 3D panicle imaging to normalize the panicle-level photosynthetic measurements. We observed differential responses in both panicle and flag leaf for two temperate Japonica rice genotypes (accessions TEJ-1 and TEJ-2) exposed to heat stress during early grain filling. There was a notable divergence in the relative photosynthetic contribution of flag leaf and panicles for the heat-tolerant genotype (TEJ-2) compared to the sensitive genotype (TEJ-1). CONCLUSION: The novelty of this method is the non-destructive and accurate determination of panicle area and photosynthetic parameters, enabling researchers to monitor temporal changes in panicle physiology during the reproductive development. The method is useful for panicle-level measurements under diverse environmental stresses and is sensitive enough to evaluate genotypic variation for panicle physiology and architecture in cereals with compact inflorescences.

13.
Front Plant Sci ; 13: 1026472, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36304400

RESUMO

Heat stress occurring during rice (Oryza sativa) grain development reduces grain quality, which often manifests as increased grain chalkiness. Although the impact of heat stress on grain yield is well-studied, the genetic basis of rice grain quality under heat stress is less explored as quantifying grain quality is less tractable than grain yield. To address this, we used an image-based colorimetric assay (Red, R; and Green, G) for genome-wide association analysis to identify genetic loci underlying the phenotypic variation in rice grains exposed to heat stress. We found the R to G pixel ratio (RG) derived from mature grain images to be effective in distinguishing chalky grains from translucent grains derived from control (28/24°C) and heat stressed (36/32°C) plants. Our analysis yielded a novel gene, rice Chalky Grain 5 (OsCG5) that regulates natural variation for grain chalkiness under heat stress. OsCG5 encodes a grain-specific, expressed protein of unknown function. Accessions with lower transcript abundance of OsCG5 exhibit higher chalkiness, which correlates with higher RG values under stress. These findings are supported by increased chalkiness of OsCG5 knock-out (KO) mutants relative to wildtype (WT) under heat stress. Grains from plants overexpressing OsCG5 are less chalky than KOs but comparable to WT under heat stress. Compared to WT and OE, KO mutants exhibit greater heat sensitivity for grain size and weight relative to controls. Collectively, these results show that the natural variation at OsCG5 may contribute towards rice grain quality under heat stress.

14.
Pest Manag Sci ; 78(10): 4037-4047, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35638857

RESUMO

BACKGROUND: To improve droplet deposition rates at the base of rice, an electrical vortex air-assisted spraying system for small- and medium-sized high-clearance boom sprayers was developed. This system uses vortex airflows to guide droplets to the base of rice and the back of leaves, as well as to increase leaf perturbation and droplet penetration and deposition. RESULTS: The spatial distribution of the airflow field generated by this system and the effects of the canopy on the airflow field were described. An orthogonal experiment was performed in a rice field based on fan speed, auxiliary airflow angle, and spray height as the experimental factors. It was discovered that a fan speed of 4000 rpm, auxiliary airflow angle of 0°, and spray height of 30 cm were optimal for droplet deposition at the base of the canopy. These settings resulted in droplet coverage of 54.5% and 35.9% on the front and back of the leaves, respectively, which are 48% and 104% higher than that on the front and back sides of leaves without an auxiliary airflow, respectively. CONCLUSION: Compared with the traditional application method, vortex air-assisted application significantly improved the rate of droplet coverage in rice canopy of different area. Hence, vortex air-assisted application enables new approaches and methods for rice crop protection. © 2022 Society of Chemical Industry.


Assuntos
Oryza
15.
BMC Psychiatry ; 22(1): 248, 2022 04 08.
Artigo em Inglês | MEDLINE | ID: mdl-35395781

RESUMO

BACKGROUND: Inflammation and immune status are correlated with the severity of major depressive disorder (MDD).The purpose of this study was to establish an optimization model of peripheral blood parameters to predict the severity of MDD. METHODS: MDD severity in the training and validation cohorts (n = 99 and 97) was classified using the Hamilton Depression Scale, Thirty-eight healthy individuals as controls. Significant severity-associated factors were identified using a multivariate logistic model and combined to develop a joint index through binary logistic regression analysis. The area under the receiver operating characteristic curve (AUC) was used to identify the optimal model and evaluate the discriminative performance of the index. RESULTS: In the training cohort, lower CD4+/CD8+ T cell ratio, albumin level, and a higher monocyte percentage (M%) were significant as operating sociated with severe disease (P < 0.05 for all). The index was developed using these factors and calculated as CD4+/CD8+ T cell ratio, albumin level, and M%, with a sensitivity and specificity of 90 and 70%, respectively. The AUC values for the index in the training and validation cohorts were 0.85 and 0.75, respectively, indicating good discriminative performance. CONCLUSION: We identified disease severity-associated joint index that could be easily evaluated: CD4+/CD8+ T cell ratio, albumin level, and M%.


Assuntos
Transtorno Depressivo Maior , Albuminas , Linfócitos T CD4-Positivos , Linfócitos T CD8-Positivos , Transtorno Depressivo Maior/diagnóstico , Humanos , Monócitos
16.
Cancers (Basel) ; 14(7)2022 Mar 24.
Artigo em Inglês | MEDLINE | ID: mdl-35406426

RESUMO

As the most lethal major cancer, pancreatic cancer is a global healthcare challenge. Personalized medicine utilizing cutting-edge multi-omics data holds potential for major breakthroughs in tackling this critical problem. Radiomics and deep learning, two trendy quantitative imaging methods that take advantage of data science and modern medical imaging, have shown increasing promise in advancing the precision management of pancreatic cancer via diagnosing of precursor diseases, early detection, accurate diagnosis, and treatment personalization and optimization. Radiomics employs manually-crafted features, while deep learning applies computer-generated automatic features. These two methods aim to mine hidden information in medical images that is missed by conventional radiology and gain insights by systematically comparing the quantitative image information across different patients in order to characterize unique imaging phenotypes. Both methods have been studied and applied in various pancreatic cancer clinical applications. In this review, we begin with an introduction to the clinical problems and the technology. After providing technical overviews of the two methods, this review focuses on the current progress of clinical applications in precancerous lesion diagnosis, pancreatic cancer detection and diagnosis, prognosis prediction, treatment stratification, and radiogenomics. The limitations of current studies and methods are discussed, along with future directions. With better standardization and optimization of the workflow from image acquisition to analysis and with larger and especially prospective high-quality datasets, radiomics and deep learning methods could show real hope in the battle against pancreatic cancer through big data-based high-precision personalization.

17.
Sensors (Basel) ; 21(24)2021 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-34960287

RESUMO

High-throughput, nondestructive, and precise measurement of seeds is critical for the evaluation of seed quality and the improvement of agricultural productions. To this end, we have developed a novel end-to-end platform named HyperSeed to provide hyperspectral information for seeds. As a test case, the hyperspectral images of rice seeds are obtained from a high-performance line-scan image spectrograph covering the spectral range from 600 to 1700 nm. The acquired images are processed via a graphical user interface (GUI)-based open-source software for background removal and seed segmentation. The output is generated in the form of a hyperspectral cube and curve for each seed. In our experiment, we presented the visual results of seed segmentation on different seed species. Moreover, we conducted a classification of seeds raised in heat stress and control environments using both traditional machine learning models and neural network models. The results show that the proposed 3D convolutional neural network (3D CNN) model has the highest accuracy, which is 97.5% in seed-based classification and 94.21% in pixel-based classification, compared to 80.0% in seed-based classification and 85.67% in seed-based classification from the support vector machine (SVM) model. Moreover, our pipeline enables systematic analysis of spectral curves and identification of wavelengths of biological interest.


Assuntos
Redes Neurais de Computação , Oryza , Análise Espectral , Máquina de Vetores de Suporte
18.
Plant Methods ; 17(1): 107, 2021 Oct 16.
Artigo em Inglês | MEDLINE | ID: mdl-34656139

RESUMO

BACKGROUND: The characteristics of light source have an important influence on the measurement performance of canopy reflectance spectrometer. The size of the effective irradiation area and the uniformity of the light intensity distribution in the irradiation area determine the ability of the spectrometer to express the group characteristics of the measured objects. METHODS: In this paper, an evaluation method was proposed to theoretically analyze the influence of the light intensity distribution characteristics of the light source irradiation area on the measurement results. The light intensity distribution feature vector and the reflectance feature vector of the measured object were constructed to design reflectance difference coefficient, which could effectively evaluate the measurement performance of the canopy reflectance spectrometer. By using self-design light intensity distribution test system and GreenSeeker RT100, the evaluation method was applied to evaluate the measurement results. RESULTS: The evaluation results showed that the vegetation indices based on the arithmetic average reflectance of the measured object could be obtained theoretically only when the light intensity distribution of the light source detected by the spectrometer was uniform, which could fully express the group characteristics of the object. When the light intensity distribution of the active light source was not uniform, the measure value was difficult to fully express the group characteristics of the object. And the measured object reflectance was merely the weighted average value based on the light intensity distribution characteristics. CONCLUSIONS: According to the research results of this paper, sunlight is the most ideal detection light source. If the passive light source spectrometer can improve the measurement method to adapt to the change of sunlight intensity, its measurement performance will be better than any active-light spectrometer.

19.
Plant Physiol ; 187(3): 1149-1162, 2021 11 03.
Artigo em Inglês | MEDLINE | ID: mdl-34618034

RESUMO

Water deficit during the early vegetative growth stages of wheat (Triticum) can limit shoot growth and ultimately impact grain productivity. Introducing diversity in wheat cultivars to enhance the range of phenotypic responses to water limitations during vegetative growth can provide potential avenues for mitigating subsequent yield losses. We tested this hypothesis in an elite durum wheat background by introducing a series of introgressions from a wild emmer (Triticum turgidum ssp. dicoccoides) wheat. Wild emmer populations harbor rich phenotypic diversity for drought-adaptive traits. To determine the effect of these introgressions on vegetative growth under water-limited conditions, we used image-based phenotyping to catalog divergent growth responses to water stress ranging from high plasticity to high stability. One of the introgression lines exhibited a significant shift in root-to-shoot ratio in response to water stress. We characterized this shift by combining genetic analysis and root transcriptome profiling to identify candidate genes (including a root-specific kinase) that may be linked to the root-to-shoot carbon reallocation under water stress. Our results highlight the potential of introducing functional diversity into elite durum wheat for enhancing the range of water stress adaptation.


Assuntos
Adaptação Fisiológica , Introgressão Genética , Estresse Fisiológico , Triticum/fisiologia , Desidratação , Secas , Variação Genética , Fenótipo , Raízes de Plantas/genética , Raízes de Plantas/crescimento & desenvolvimento , Raízes de Plantas/fisiologia , Brotos de Planta/genética , Brotos de Planta/crescimento & desenvolvimento , Brotos de Planta/fisiologia , Triticum/genética , Triticum/crescimento & desenvolvimento
20.
IEEE Comput Graph Appl ; 41(5): 57-66, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34280091

RESUMO

It is challenging to interpret hyperspectral images in an intuitive and meaningful way, as they usually contain hundreds of dimensions. We develop a visualization tool for hyperspectral images based on neural networks, which allows a user to specify the regions of interest, select bands of interest, and obtain hyperspectral classification results in a scatterplot generated from hyperspectral features. A cascade neural network is trained to generate a scatterplot that matches the cluster centers labeled by the user. The inferred scatterplot not only shows the clusters of points, but also reveals relationships of substances. The trained neural network can be reused for time-varying hyperspectral data analysis without retraining. Our visualization solution can keep domain experts in the analytical loop and provide an intuitive analysis of hyperspectral images while identifying different substances, which are difficult to be realized using existing hyperspectral image analysis techniques.


Assuntos
Redes Neurais de Computação
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